Membrane expression of CD16 (PE anti-human CD16), CD62L
(FITC anti-human CD62L
) on neutrophils was assessed by flow cytometry.
Briefly, samples (20 [micro]L) were allocated in tubes containing 100 [micro]L PBS and incubated with a 10 [micro]L antibody solution specific for CD3 FITC-anti-mouse, CD4 PerCP-Cy5,5-anti mouse, CD8 PE-anti mouse, CD14 FITC-anti mouse and CD 14 PEanti mouse, HLA-DR FITC anti-mouse, CD11b PerCP-Cy5,5-anti mouse, CD19 PE-anti mouse, CD45 PerCP-Cy5,5-anti mouse, CD56 PerCP-Cy5,5-anti mouse, and CD62L
FITC anti-mouse (all from BD Pharmingen, San Diego, CA, USA) in the dark for 30 min.
(L-selectin) CD134 (OX40) IL-2 receptor alpha chain (CD25) etc.
Altered gene transcription mediated by glucocorticoid- bound GR could explain reduced expression of certain plasma membrane proteins on neutrophils, such as CD62L
that is severely down-regulated in cows at parturition (Lee and Kehrli, 1998).
Nenhuma diferenca na expressao de CD62L
(P = 0,24), CD11b (P = 0,21) e CD44 (P = 0,16) foi observada.
Based on recent studies showing in vivo antioxidant effects of thymoquinone (TQ), the active ingredient of Nigella sativa seeds, this study aims to determine whether or not TQ can increase survival and sustain the expression of the homing receptor CD62L
in antigen-specific T cells in vitro.
The SHML histiocytes express monocyte/macrophage-associated antigens (CD68, HAM56, CD14, and CD64) and adhesion molecules (CD11b, CD11c, CD18, CD62L
, and CD103).