is an adequate tissue fixative for routine surgical pathology, preserving cell morphology and molecular integrity.
Treatment options that have been used for KCOT include marsupialization, enucleation and curettage, and enucleation with chemical cautery (Carnoy's solution
), thermal (cryotherapy), or mechanical (peripheral ostectomy) cautery of surrounding tissue and osseous resection with or without continuity defect.
Thereafter, the roots of the plants were cut and treated with Carnoy's solution
1 consisting of ethanolacetic acid (3: 1) for 30 min at room temperature followed by washing with distilled water.
Lau et al reported recurrence rates of 3.6% for resection, 30.5% for enucleation alone, 16% for enucleation followed by Carnoy's solution
application, and 18% by marsupialization followed by enucleation.
Harvested cells were treated with 75 mM KC1 for 20 min and subsequently fixed with 5 mL Carnoy's solution
. Cells were fixed onto prewetted glass slides and dried on a 40[degrees]C plate.
Gay-Escoda, "Role of Carnoy's solution
in the treatment of keratocystic odontogenic tumor: a systematic review," Medicina oral, patologia oral y cirugia bucal, vol.
In conservative treatment regimens Carnoy's solution
was applied after enucleation of the tumour whereas the patients of aggressive surgery were operated with minimum 5mm safety marginal clearance of the tumour.
This smear was air dried and fixed in Carnoy's solution
(methanol and glacial acetic, 3:1) for 2 hours.
Aggressive treatment addresses the "neoplastic nature" of the KCOT and includes peripheral ostectomy, chemical curettage with Carnoy's solution
or en bloc resection.
To maintain the morphology, specimen-pins were inserted completely through the uterus and fetal membranes at the level of the external intercomual bifurcation, cross-sections (0.5-cm thick) were taken, and tissues were immersion-fixed in Carnoy's solution
and embedded in paraffin.
All animals were fixed in Bonin's liquid (15:4:1 of picric acid saturated solution, formaldehyde 40% and acetic acid) or Carnoy's solution
(6:3:1 of ethanol, chloroform and acetic acid) for 2 hours at 20 [degrees]C, dehydrated in ethanol and embedded in paraffin.