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Progressive loss of efficiency and fatal outcome resulting in massive economical losses forced veterinary authorities worldwide to start disease control including mass testing using complement fixation test and/or malleinisation, and culling of positives.
25 Table 2--Kappa index of enzyme-linked immunosorbent assay (ELISA-C) indirect ELISA (I-ELISA) and fluorescence polarization test (FPA) according to the gold standard based on the combination of complement fixation test and 2- mercaptoethanol test for diagnosis of brucellosis in buffaloes.
The virus was quickly recognized as an influenza A virus by complement fixation tests.

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