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Fully phased consensus sequences were generated for each allele and exported from Twin[TM] in FASTA format.
In this study, we obtained a 429-bp fragment from a novel splicing variant of FAMLF, and confirmed that the 1-363 bp sequence was the consensus sequence of known FAMLF splicing variants by comparing it with other FAMLF transcripts (EF413001.1 and NR_040073.1).
Part of the bioinformatics data associated with the tiled consensus sequence generated is a measure of certainty, or quality, at each nucleotide position to give the end user some sense of the level of statistical certainty for each base position.
The oligonucleotides (MWG Biotech AG) were designed in order to clone the lexA box consensus sequence into the pBR322 vector upstream the b/aTEM-i reporter gene.
The next step of the probe design strategy consists in incrementing a window of length "l" (l is the length of probes set by the user) along the consensus sequence to find all possible degenerate probes that do not contain gaps ("-") and whose degeneracy does not exceed the threshold value of maximum degeneracy allowed.
CAAT###CAATBOX1###20###10 15 21 18###Promoter consensus sequence was found in legA seed storage protein (Shirsat et al.,
"The human genome reference is a consensus sequence, which is a politically wonderful outcome.
A consensus sequence, obtained with Clustal X (Thompson et al., 1994), was then deduced from the alignment of these 21 sequences.
Consensus sequence GQC, KGHYTEG, DNE AL and glycine rich sequences SLGGGTGSG MG are binding site for phosphoryl group of nucleotide and are observed in other nucleotide binding proteins (Figure 1) (22).
After spending $3 billion and nearly 15 years, the researchers working on the Human Genome Project developed a consensus sequence of one human genome.
The software was used to assemble each forward and reverse sequence into a consensus sequence, which was edited to resolve base pair ambiguities between the two strands by evaluation of electropherograms.
In this study we identified "young" B2 elements from the GenBank database based on their sequence identity to a consensus sequence (Kass et al.