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This indicates that, Taq DNA polymerase is substantially susceptible to error-prone DNA amplification. It is shown that, mutations in proteins with N-terminal deletion lead to reduced tendency toward errors, simillarly several recent studies reported about the latest discoveries on the thermo-stable DNA polymerase with an integral editing exonuclease activity [4].
Thus, the direct PCR approach reported here is a new alternative for DNA amplification from FAW samples.
The most advanced product in X-Pol portfolio is Qualiphi, which is believed to increase the performance of DNA amplifications significantly.
DNA amplification methods for diagnosis and epidemiological investigations of avian mycoplasmosis.
DNA amplification. Amplification conditions were conducted according to the protocol suggested by Kalmar et al.
Detection of Trypanosoma cruzi by DNA amplification using the polymerase chain reaction.
According to the research team, led by Boston University Biomedical Engineering Associate Professor Amit Meller, the method could eliminate the expensive, time-consuming and error-prone step of DNA amplification.
She comes with an extensive laboratory and molecular biology background that includes measuring enzymatic activities (Enzyme Assays), measuring the concentration of substances in biological liquids (Immunoassays), DNA amplification via polymerase chain reaction (PCR) or recombinant DNA techniques, Gravimetric Analysis and Potentiometric and pH Titration measurement.
Store the samples in the freezer or on ice if continuing with the DNA amplification.
Cepheid's easy-to-use systems integrate a number of complicated and time-intensive steps, including sample preparation, DNA amplification and detection, which enable the analysis of complex biological samples in its proprietary test cartridges.
(1991) were employed, as were those involving DNAzol (Gibco BRL, Gaithersburg, MD, USA), the rapid genomic blood DNA amplification kit (Pharmacia Biotech, Piscataway, NJ, USA), Chelex-100 (Bio-Rad, Hercules, CA, USA), and the QIAamp DNA Mini Kit (QIAgen, Valencia, CA, USA) (Alves et al.