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Primary explant cultures of gill and heart tissues from the abalone Haliotis asinina were undertaken using Leibovitz L15 medium supplemented with IGF-2 and basic fibroblast growth factor.
Tissues were rinsed in cold phosphate-buffered saline (PBS) to remove excessive blood and processed for explant cultures within 2 hr.
In conclusion, findings of this study, showed that oral epithelial cells can be cultured in vitro by explant culture method similar to limbal epithelial cells.
In this regard, the highest concentration of fetal DNA was detected in STBM prepared by in vitro vinous explant cultures (Fig.
Therefore, optimization of culture method and conditions is necessary for the derivation of ES cells by whole explant culture using in vitro produced embryos.
This suggests that the explant culture system used in the present studies is unable to faithfully reproduce the main effects of DBP/MBP on steroidogenesis that are evident in vivo.
An additional significant finding of current study, which may have clinical implications, is that cyclooxygenase inhibitors augment LTB4 production by OA explant cultures.
2] + RU486) were used to test for effects of treatments in the explant cultures.