The report investigates technologies to enhance the efficacy of antibodies or to make them safer drugs and specifically analyzes five key antibody technologies: antibody drug conjugates, Fab fragments
, Fc engineering/glycoengineering, bispecific antibodies, and radioimmunotherapies.
Reaction volume, amount of the tracer molecules, and mixing efficiency were varied to test whether the smaller size of the Fab fragment compared with the mAb had an effect on assay kinetics.
The reduced size and the site-specificity of the Fab fragment provided higher surface density than an intact antibody, indicating improved assay performance.
The signals obtained after subtracting the nonspecific binding from the total binding were used to calculate the affinity of the labeled Fab fragment
Utilization of recombinant fab fragments
in a cTnI immunoassay conducted in spot wells.
are derived from human antibodies and can be manufactured as separate recombinant proteins.
Incubation with antigen-digoxigenin was performed for 1 hr at 37[degrees]C, and after washing, 100 [micro]L/well peroxidase-conjugated sheep anti-digoxigenin Fab fragments
(Boehringer) diluted 1:3,000 in PBS/BSA-Tween 20 was added.
LambdaFabSelect enables bioprocess engineers to use BAC's ligand technology to produce Fab fragments
with a lambda light chain for clinical and commercial use.
mutation of the complementarity-determining regions of scFv or Fab fragments
Digoxin measurements following plasma ultrafiltration in two patients with digoxin toxicity treated with specific Fab fragments
From crystallographic data it is known that an IgG antibody (MT 146 000) is arranged in three discrete domains: two Fab fragments
and one Fc (30-32).
Administration of Fab fragments
has successfully been used to reverse the effects of life-threatening digoxin overdoses for over 20 years.