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Feulgen stain was drained and the root tips were rinsed with cold double distilled water and stained with Carbol fuchsin stain overnight in a refrigerator.
For mitotic analysis, root tips were hydrolyzed in 1 M HC1 at 60 [degrees] C for 10 min, rinsed in distilled water and transferred to Feulgen stain for 30 min in dark.