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2014), one must look at the function of inner medullary thin limbs of Henle's loop, collecting ducts, and vasa recta to understand how the inner medullary osmotic gradient is generated.
It was shown more than five decades ago in studies of how the corticomedullary osmotic gradient is generated, that luminal fluid near the bend of thin limbs of Henle's loop of hamster, kangaroo rat, and sand rat is nearly isoosmotic with fluid of the inner medullary interstitium, collecting ducts, and blood vessels at the same level.
The water channel AQP1 is the predominant pathway by which water is reabsorbed from the descending thin limb of Henle's loop in the process of urine concentration (Chou et al.