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It has been difficult to determine the phylogeny of the genes that encode Fe-hydrogenase and PFOR, because these iron-sulfur proteins are absent from mitochondria, [alpha]-proteobacteria, and most other eubacteria.
In humans, most of the iron is contained within the porphyrin ring of heme in proteins such as hemoglobin, myoglobin, catalase, peroxidases, and cytochromes, [3] as well as iron-sulfur proteins, such as NADH dehydrogenase and succinate dehydrogenase, in which iron is present in clusters with inorganic sulfur.