After the addition of anti-TNF-[alpha] to M-CSF
+ RANKL treatment, the observed differences in the erosion profile between the three groups were lost (Figure 2(b)).
Zlotnik, "Biological role of granulocyte macrophage colony-stimulating factor (GM-CSF) and macrophage colony-stimulating factor (M-CSF
) on cells of the myeloid lineage," Journal of Leukocyte Biology, vol.
IL: interleukin; MMP: matrix metalloproteinase; M-CSF
: macrophage colony-stimulating factor.
Osteoclast precursors were treated with macrophage colony-stimulating factor (M-CSF
) alone, M-CSF
with RANKL, M-CSF
with RANKL and exendin-4, and M-CSF
with exendin-4 for 5 days, then stained with TRAP solution.
(a) BMMs were incubated in 10% FBS-[alpha]-MEM containing M-CSF
(30 ng/ml) and liensinine or nuciferine at various concentrations for 5 days.
Western blot analysis indicated that sanguiin H-6 strongly inhibited the nuclear translocation of NFATc1, phosphorylated-c-Fos, and NF-[kappa]B 6 and 24 h after M-CSF
and RANKL treatment, although M-CSF
and RANKL markedly activated these transcription factors (Fig.
and its receptor are potential therapeutic targets in mechanical stress-induced odontoclastogenesis, and injection of an anti-c-Fms antibody might be useful to prevent mechanical stress-induced root resorption during orthodontic tooth movement.
: a novel plasmacytoid and conventional dendritic cell poietin," Blood, vol.
Splenocytes were cultured in methylcellulose-based medium (StemCell Technologies, Vancouver, British Columbia, Canada) supplemented with M-CSF
(30 ng/mL) for 12 days.
is an important growth factor involved in the development of specific cells which are involved in the immune responses in the lungs.
The fact that osteoblasts secrete two major cytokines, namely, macrophage colony stimulating factor (M-CSF
) and granulocyte-macrophage colony stimulating factor (GM-CSF) led this group to believe that recombinant BMP-2 may play a major role in hematopoiesis, and further, serve as a potential cytokine in stem cell transplantation.