Since p53 GOF mutant protein
is a misfolded protein, proteasome inhibition may induce cell death through excessive accumulation of misfolded proteins.
This study showed that immunohistochemistry with SP174 antibody allowed identification of KRAS and NRAS Q61R mutant proteins
. Although specificity and sensitivity of this test were 100%, differentiation between KRAS Q61R and NRAS Q61R was not possible.
Meanwhile, cell immunofluorescence showed that the wild type COMP was homogenously expressed in cytoplasm after transfection for 24 hours, but, interestingly, mutant proteins
were irregularly accumulated inside the cell membrane (Figure 4(b)).
To examine the mode of binding, binding stability and interaction profiles of ligand and receptor proteins, all mutant proteins
were subjected to docking with Helicoverpa armigera Cadherin and APN receptors and Aedes aegypti ALP by clusPro(2.0) software.
All malignant tumors harbor genetic alterations, some of which may lead to the production of mutant proteins
that are capable of triggering an antitumor immune response.
From the RMSD results of both wild and mutant protein
structures, the mutant protein
structure shows more stability when compared to the wild type protein structure.
The dimeric form of CTLA-2[alpha] was no longer detected by SDS-PAGE of such mutant proteins
, suggesting that the dimeric form of CTLA-2[alpha] resulted from the formation of an intermolecular disulfide bond between monomers.
Other topics of the 17 chapters include carbohydrate-based vaccines, functional mutant proteins
, nanotechnology for cancer screening and diagnosis, small interfering RNAs, gold nanoparticles, embolization devices from biomedical polymers, and 3D cancer tumor models for evaluating chemotherapeutic efficacy.
"Our model revealed that increasing another cell chemical called progranulin reduced the death of neurons by combating the accumulation of the mutant proteins
. Furthermore, this approach may protect against neurodegenerative diseases other than Huntington's disease," he noted.
Mutagenesis and Expression of Mutant Proteins
: Cr y1Ac mutants, H168Q and H168R, were prepared as described earlier (Hussain et al., 2010).
The major target of the active C3bBb is C3 itself, resulting in rapid amplification of the complement cascade.To capture structural snapshots of C3bB and C3bBD, the researchers first generated mutant proteins
that would stabilize the complexes in their active forms.
Scientists identified the gene that generates "mutant proteins
" responsible for killing nerve cells.