Table 1: Zeta Potential of and the cytotoxicity, hemolysis, C3 depletion and platelet factor 4
(PF4) on incubation with rasa chenduram and naga parpam Zeta potential Cell Viability Hemolysis (mV) (%) (%) Control -- -- -- Rasa chenduram -35.7 [+ or -] 1.06 40 [+ or -] 5 2.47 Naga parpam -32.6 [+ or -] 0.46 75 [+ or -] 5 0.34 C3 concentration PF4 concentration (mg%) (IU/ml) Control 127 7.5 [+ or -] 0.50 Rasa chenduram 125 18.4 [+ or -] 1 Naga parpam 126 7.5 [+ or -] 0.50 Table 2: Elemental analysis of rasa chenduram and naga parpam by EDAX Element Rasa chenduram Naga parpam Fe 11.10 4.68 Hg 88.91 0 Zn 0 95.32
Other negative/unremarkable studies included platelet factor 4
antibody, D-dimer, fibrinogen, serum immunoglobulins, Epstein-Barr virus polymerase chain reaction, and thyroid function tests.
In heparin-induced thrombocytopenia (HIT), unfractionated standard heparin binds platelet factor 4
(PF4), a heparin-neutralizing protein synthesized by megakaryocytes and stored in platelets.
In vitro platelet activation has consequences for the evaluation of heparin activity as well (4) because activated platelets might release platelet factor 4
(PF4), inactivating heparin and leading to artifactual shortening of activated partial thromboplastin time (APTT) results.
[Alpha] granules contain a number of different proteins, including fibrinogen, platelet-derived growth factor, von Willebrand factor, factor V, fibronectin, [Beta]-thromboglobulin, and heparin-neutralizing factor (platelet factor 4
).[1,2] [Delta] Granules contain calcium, serotonin, pyrophosphate, adenosine diphosphate, and adenosine triphosphate.[1,2] Determination of storage pool organelles by transmission electron microscopy allows the identification of storage pool defects.
Immuno-globulin G from patients with heparin-induced thrombocytopenia binds to a complex of heparin and platelet factor 4
1) The [alpha] granules contain platelet thrombospondin, fibrinogen, fibronectin, platelet factor 4
, von Willebrand factor (VWF),  platelet-derived growth factor, [beta]-thromboglobulin, and coagulation factors V and VIII.
The use of citrate-theophylline-adenine-dipyridamol (CTAD) buffer rather than citrate decreased platelet activation as indicated by lowering the plasma concentrations of platelet factor 4
Platelet activation is measurable by quantifying thromboxanes, platelet factor 4
, and thrombomodulin (TM).
Laboratory-based tests such as aggregometry, adhesion tests, viscometry, flow cytometry, electron microscopy, secretion assays, and measurements of platelet activation markers such as [beta]-thromboglobulin, platelet factor 4
, or thromboxane [A.sub.2], can provide useful information.