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Eyestalk reinjection into ESL crabs diminished the degree of ESL-stimulated CAc mRNA induction in G7 by 3-fold; however, the level of gene expression was still significantly above that of 35-ppt intact crabs by 33-fold (P < 0.05, Student's r-test) and not significantly different from that of noninjected ESL crabs (Fig.
R-test comparison of four conditions (prefatigue, PreFat; post-fatigue, PostFat; PreRest and PostRest) showed a significant shape difference for the CE response within conditions, between axes in all cases, except the PostFat state.
In this approach to statistical comparison of group CE responses, we perform the K-L analysis and R-test to determine if the shape of the CE response is similar between groups.