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These peaks were thought to be potentially related to 3 S100 proteins: S100A8 (calculated mass, 10 835 Da), S100A9 (calculated masses, 12 691 Da and 13 242 Da), and S100A12 (calculated mass, 10 444 Da).
We confirmed the identities of the S100A8, S100A9, and S100A12 proteins by comparing WB and SELDI-TOF MS results for the serum samples (Fig.
Residues located near the C-terminus of S100A12 are predicted to mediate S100A12-receptor recognition (40).