30] made
serial passages of the virus in chicken embryos resulting in adaptation of the virus and attenuation of very virulent and classic strains.
After 1
serial passage in PrP-b (VM) mice, the sample from the goat with suspected BSE generated IP of 128 [+ or -] 4 (mean [+ or -] SD) days postinoculation similar to serially passaged ovine BSE (109 [+ or -] 4) and the 301V mouse-adapted BSE strain (107 [+ or -] 6).
Multiple
serial passage experiments conducted by PolyMedix and others on PMX-30063 and related PolyMedix antibiotic compounds support our view of a lower likelihood of developing resistance.
At second
serial passage of bovine TME, L-type BSE, and typical BSE into TgOvPrP4 mice, the survival periods were shortened for all 3 sources, but especially for L-type BSE (202 [+ or -] 26 days) and bovine TME (234 [+ or -] 27 days), although survival periods were statistically different for these 2 groups of mice (p = 0.
In that study investigators failed to observe an increase in viral shedding from symptomatic piglets upon
serial passage, despite successful intracellular detection of viral RNA and newly synthesized virus-encoded protein in host cells dying of apoptosis.
We are delighted by BIO's recognition of Koronis' innovative approach for the treatment of HIV based on Viral Decay Acceleration(TM), a novel mechanism of action that was recently revalidated in a third
serial passage study," said Donald Elmer, interim Chief Executive Officer of Koronis Pharmaceuticals.
Multiple
serial passage experiments conducted by PolyMedix and others on PMX-30063 and related PolyMedix antibiotic compounds also support our view of a lower likelihood of developing resistance.
Serial passage of the virus on Vero cells to obtain a tertiary viral stock yielded 9.
After
serial passage, 8 of 10 strains developed cefoxitin resistance, none developed promoter or attenuator mutations, 6 lost both the OmpC and OmpF porin proteins, and 1 showed decreased production of both.
Plasmid stability of screened quinolone resistant isolates as well as their transformants were analyzed by
serial passages method for consecutive 75 days at 1: 1000 dilutions without any antibiotic pressure (6).
