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Pellacani) evaluated the RCM images, which showed a representative horizontal section of the epidermis at the stratum spinosum, while the two dermatopathologist raters evaluated photomicrographs taken at histopathology examination, grading the keratinocyte atypia they observed.
Histopathological examination showed partial cytoplasmic vacuolisation, and several cells with eosinophilic cytoplasmic inclusions were observed in the stratum spinosum layer of the stratified squamous epithelium.
Images were taken of the cheek and the outer surface of volar forearm and measurements were made of stratum corneum (SC) thickness, stratum granulosum (SG) thickness and stratum spinosum (SS) thickness.