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To collect a sample the lesion must be unroofed using a Dacron swab, which is then placed in a viral transport medium and processed within 24 hours.
NPA samples were collected by suction into a disposable aspiration trap containing 3 mL of viral transport medium (containing, per liter, 2 g of sodium bicarbonate, 5 g of bovine serum albumin, 200 [micro]g of vancomycin, 18 [micro]g of amikacin, and 160 U of nystatin in Earle's balanced salt solution).
Urine was collected directly from the bat's urogenital opening, before the blood sampling, with a cotton swab that was immediately placed in a cryotube containing 1 mL viral transport medium.