Lu, "Adherent cells
in granulocyte-macrophage colony-stimulating factor-induced bone marrow-derived dendritic cell culture system are qualified dendritic cells," Cellular Immunology, vol.
, such as mesenchymal stem cells (MSCs), are cultured on treated plastic surfaces and must adhere to these surfaces in order to grow.
Developed by Horacio Espinosa and John Kessler, the localized electroporation device (LEPD) can be applied to adherent cells
, which are grown on an artificial substrate as opposed to free-floating in a culture medium and are able to continue growing and differentiating.
The new method, which has been developed using Vera adherent cells
attached to Cytodex 1 microcarriers, allows a 20 percent media exchange to be performed at any time of the day or night on 24 ambr vessels in approximately 4h.
3B) anoikis-resistant cells through matrigel-coated transwell chambers was approximately 50% greater than that of their adherent cell
Hoffmeister, H., Sponceram[R] Carrier Discs for Adherent Cell
Culture, Rev 103, ZELLWERK GmbH, Germany, 2008, pp 1-2.
Key features of the assay include accurate and precise data; mix-and-read protocols that allow for rapid measurement and instantaneous results; a proprietary combination of two fluorescent dyes that discriminate viable from dead, nucleated cells; and validation on a variety of both suspension and adherent cell
"In order to use the cellexis technology, the use of so-called semi-adherent or adherent cell
lines is required, i.e.
The new space is completely operational and will be used for cell culture labs, capacity for adherent cell
scale-up of 10 billion cells, controlled rate freezer capable of cryopreserving 600 vials of cells, capability for membrane preparations of up to two gram of protein, large-scale electroporator capable of generating three billion transfected cells, flow sorter, dedicated molecular biology lab and Radioisotope lab, which is to be completed later this month.
“Our design objective was to combine the best of suspension cultivation with the advantages of adherent cell
Proprietary angled dispensing ensures compatibility with all dispense protocols from gentle treatment of loosely adherent cell
monolayers to rigorous or viscous reagent additions.
The Cellaxess Elektra opens a new dimension in RNAi delivery, high-throughput screening and electroporation of primary adherent cell
types," said Dr.