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We tried to culture cells adherently in this stage to avoid damaging the dissociated spheres in the next stage; however, the ratio of positive labeling decreased.
Adherently growing cells were allowed to attach overnight and treated after 24 h.
Human breast adenocarcinoma cell lines MDA-MB 231 and BT 474 were grown adherently and maintained in Dulbecco's Modified Eagle's Medium supplemented with 100 U/mL penicillin, 100 [micro]g/mL streptomycin, and 10% fetal bovine serum (FBS).
The rust layer was turned into needle-like shape and absorbed adherently and compactly when the hydrostatic pressure was 0.4 MPa.
Human anterior lens capsule-lens epithelial cells (aLC-LECs) from cataract surgery and fibrovascular epiretinal membranes (fvERM) from proliferative diabetic retinopathy (PDR) were cultured adherently under viscoelastic material.