50 [micro]L of each sample was
aliquoted for Lowry protein assay.
The supernatant was
aliquoted in 100-[micro]L portion, snap-frozen in liquid nitrogen, and then stored at -80[degrees]C until use.
In prenatal testing, samples should be
aliquoted and stored frozen for comparison with subsequent samples.
Although manufacturers state that their 96-well products are stable if kept at constant refrigeration, this may not always be possible unless reagents are initially
aliquoted and there after used one-aliquot-at-a-time.
Experimental design: The sperm samples were pooled and
aliquoted into three parts.
Then, the supernatants were collected,
aliquoted, and stored frozen at [?
An
aliquoted FOXL2 PCR product was used for bidirectional DNA sequencing.
At the selected time point, 100 [micro]l of the ALP (exposed to heavy metal and UV) was
aliquoted out and added to a cuvette containing the Tris buffer and pNPP (33.
After the treatment, the material retained by the membrane was
aliquoted in 2 mL and stored at -70[degrees]C, while the filtrated material was
aliquoted in 10 mL and also stored at -70[degrees]C, until the use.
Specimens were
aliquoted in freezer-type tubes (O-ring enclosures) and frozen at -70[degrees]C (-94[degrees]F).
Samples were centrifuged for 10 min at 10000 g and serum separated,
aliquoted and stored at -80 oC until used.
Blood sampling was performed without anticoagulant according to standard techniques and after 30 min, the tubes were centrifuged at 2,000 rpm for 5 min and then sera were
aliquoted in several labeled vials and kept frozen at -20[degrees]C.
