Red blood cells and plasma samples were separated,
aliquoted, and stored at -20[degrees]C prior to analyzing the samples as quality controls.
After centrifugation, the supernatant containing myofibrillar proteins was recovered,
aliquoted, and frozen at -80[degrees]C until further protein analysis to avoid calpain protease activation.
CSPCs were
aliquoted into 500 [micro]L DMEM/F12 of 2.5 x 10 [and] 5 cells.
The amount of sample being
aliquoted is set in the TSM, or Total System Manager according to the needs of the testing laboratory, but typically averages about V ml.
The RNA was extracted from liver samples with the Trizol reagent and then
aliquoted. RNA was quantified using the Qubit fluorimeter (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA).
Gentle shaking ensures homogenisation before portions of 2 ml are
aliquoted into sterile dropper bottles.
100 [micro]L of cell lysate was
aliquoted for protein quantification by Lowry protein assay [17] and 900 [micro]L of the remaining cell lysate was mixed and incubated with 1.35 mL ice-cold absolute ethanol for 10 min.
Both Ad-TSHR289 and Ad-Lacz in this study were obtained from the same preparation,
aliquoted and stored at −80[degrees]C.
Cell suspension was
aliquoted in four 15 ml test tubes with 20 x [10.sup.4] cells per tube.
Plasma samples are sensitive to freeze-thaw cycles and should be
aliquoted (50) before freezing.
Eventually, these primers will be
aliquoted in PCR micro-plate arrays for rapid and simultaneous identification of multiple Salmonella serovars.
The mouse embryonic fibroblasts were
aliquoted into four parts.
