Red blood cells and plasma samples were separated, aliquoted
, and stored at -20[degrees]C prior to analyzing the samples as quality controls.
After centrifugation, the supernatant containing myofibrillar proteins was recovered, aliquoted
, and frozen at -80[degrees]C until further protein analysis to avoid calpain protease activation.
CSPCs were aliquoted
into 500 [micro]L DMEM/F12 of 2.5 x 10 [and] 5 cells.
The amount of sample being aliquoted
is set in the TSM, or Total System Manager according to the needs of the testing laboratory, but typically averages about V ml.
The RNA was extracted from liver samples with the Trizol reagent and then aliquoted
. RNA was quantified using the Qubit fluorimeter (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA).
Gentle shaking ensures homogenisation before portions of 2 ml are aliquoted
into sterile dropper bottles.
100 [micro]L of cell lysate was aliquoted
for protein quantification by Lowry protein assay  and 900 [micro]L of the remaining cell lysate was mixed and incubated with 1.35 mL ice-cold absolute ethanol for 10 min.
Both Ad-TSHR289 and Ad-Lacz in this study were obtained from the same preparation, aliquoted
and stored at −80[degrees]C.
Cell suspension was aliquoted
in four 15 ml test tubes with 20 x [10.sup.4] cells per tube.
Plasma samples are sensitive to freeze-thaw cycles and should be aliquoted
(50) before freezing.
Eventually, these primers will be aliquoted
in PCR micro-plate arrays for rapid and simultaneous identification of multiple Salmonella serovars.
The mouse embryonic fibroblasts were aliquoted
into four parts.