After 48 h of incubation at 35[degrees]C, the eggs were chilled at 4[degrees]C overnight before the allantoic fluid
Viral RNA was isolated from virus-containing allantoic fluid
with the RNeasy Mini kit (QIAGEN, Valencia, CA, USA) as specified by the manufacturer.
Our earlier data had shown that infected allantoic fluid
retained virus without significant loss for 24 h at 37[degrees]C (Jadi and others, unpublished data).
We sequenced the entire genome of the virus by using RNA harvested from the allantoic fluid
of infected eggs.
None of the nasal /ocular swabs and allantoic fluid
samples collected from chicken embryos inoculated with samples from Farm Nos.
from the second passage was used in hemagglutination testing performed on chicken erythrocytes, which resulted in a titer of 64 hemagglutinating units.
6,510,811 (the '811 patent) relates to a method for localizing the allantoic fluid
of avian eggs.
was recovered from infected eggs and stored at -80[degrees]C.
In their progeny embryos, IgA, IgG, and IgM antibodies to these Mycoplasma species were detected in the allantoic fluid
(ALF) and amniotic fluid (AMF) on day 14 of embryonic development (ED).