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After 48 h of incubation at 35[degrees]C, the eggs were chilled at 4[degrees]C overnight before the allantoic fluid was harvested.
Viral RNA was isolated from virus-containing allantoic fluid with the RNeasy Mini kit (QIAGEN, Valencia, CA, USA) as specified by the manufacturer.
Our earlier data had shown that infected allantoic fluid retained virus without significant loss for 24 h at 37[degrees]C (Jadi and others, unpublished data).
We sequenced the entire genome of the virus by using RNA harvested from the allantoic fluid of infected eggs.
None of the nasal /ocular swabs and allantoic fluid samples collected from chicken embryos inoculated with samples from Farm Nos.
Allantoic fluid from the second passage was used in hemagglutination testing performed on chicken erythrocytes, which resulted in a titer of 64 hemagglutinating units.
6,510,811 (the '811 patent) relates to a method for localizing the allantoic fluid of avian eggs.
Allantoic fluid was recovered from infected eggs and stored at -80[degrees]C.
In their progeny embryos, IgA, IgG, and IgM antibodies to these Mycoplasma species were detected in the allantoic fluid (ALF) and amniotic fluid (AMF) on day 14 of embryonic development (ED).