Larvae transport [.sup.14]C-glycine from seawater into the intracellular free
amino acid pool. The endogenous pool of non-radiolabeled glycine remains nearly constant during the relatively short period of the transport assay (~50 min); however, the ratio of [.sup.14]C-radiolabeled to non-radiolabeled glycine changes (i.e., the specific activity of glycine in the free
amino acid pool changes continuously during the transport assay).
bistortoides, as evidenced by large seasonal variations in the rhizome
amino acid pool.
The amount of glycine in the free
amino acid pool increased significantly during development (ANOVA: [F.sub.[1,46]] = 45.43, P [less than] 0.0005; [ILLUSTRATION FOR FIGURE 1B OMITTED]).
AC Caribou harvested at 600 GDD showed an increase in total N and total
amino acid pools from the time of the third harvest (7 Oct.
Unlike systems [y.sup.+]L, [b.sup.0,+] and [B.sup.0,+], system [y.sup.+] is ubiquitously expressed and functions to accumulate lysine and arginine intracellular
amino acid pools for nitrogen metabolism (Verrey et al., 2000).
During June, when extractable
amino acid pools were maximal, populations (most probable numbers, MPN) capable of growth on glycine or glutamate were also high [ILLUSTRATION FOR FIGURE 5B OMITTED].
Intracellular free
amino acid pools function in cell volume regulation (Pierce, 1982; Yancey et al., 1982; Rice and Stephens, 1988), and the primary function of taurine may be as a compatible osmolyte, a function which is conserved throughout the animal kingdom (Allen and Garrett, 1971; Huxtable, 1992).