(14) Hybridization signals for the MYCC and MYCN probes were counted in more than 100 nuclei, and the number of chromosome 9 centromeric signals was reported for each nucleus; increases in the number of signals greater than 5 were recorded as amplifications
of target cDNA with T7 RNA polymerase (1) is currently the most common method for the amplification
of RNA for microarray analysis and has been validated (2) and optimized (3, 4).
(2) However, no amplification
was observed, even after attempts to optimize the protocol.
Nonspecific products generated through primer--primer interactions may interfere with the amplification
of targets, decreasing sensitivity and selectivity.
Constitutive activation of FGFR1 occurs basically by three major mechanisms: gene amplification
, translocation, or activating mutations.
Cell-cycle alterations were common, including homozygous deletion of CDKN2A in 4 (20%) and amplifications
of CCND3, CCNE1, CCND1, CCND2, and CDK4 in 8 (40%) of the MPBC cases, a subset of which may indicate the potential for use of cell-cycle inhibitors.
2 April 2014 - US advanced cancer genome analysis and testing services provider Personal Genome Diagnostics Inc, or PGDx, said Wednesday it had introduced the METDetect Assay for the identification of MET gene amplifications
in the circulation of cancer patients.
Actually, this identification is based on genetic techniques, which involve DNA amplification
. Standard protocols include several steps of DNA extraction and purification prior to PCR amplification
of DNA, which require much labor when the number of samples is large.
ttMutS showed a significant suppressing effect on the nonspecific amplifications
in the presence of 0 to 0.4 mM ATP (Figure 1(c)).
Through the acquisition, Sygnis will shift its focus to the development and marketing of X-Pol's DNA amplification
technologies and products.
If we limited "clause" to a sequence containing a morphological verb that expresses an event or state and has not been lexicalized as a noun, then (11) would be a single clause that contained three amplifications
Detection of gene amplification
in MYCN, C-MYC, MYCL1, ERBB2, EGFR, AKT2, and human papilloma virus in samples from cervical smear normal cytology, intraepithelial cervical neoplasia (CIN I, II, III), and cervical cancer