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If speech is to be protected so that all ideas can be heard, we have to face the music and deal with the power of commercially amplified speech and its destructive influence on thoughtful discourse in this country.
4) amplified 4,682 bp of rompB and showed a high degree of nucleotide sequence similarity (99.
To identify which SFG rickettsia was responsible for the patient's febrile illness, segments of 5 rickettsial genes were amplified by PCR, and the amplicons produced were sequenced and compared to reported sequences of other SFG rickettsiae.
Amplified DNA was detected by electrophoresis in a 2% agarose gel and visualized under blue light after SYBR-Gold (nucleic acid gel stain) (Molecular Probes, Leiden, the Netherlands) staining.
RNA (5 [micro]L) was reverse transcripted and cDNA amplified with primers D1 and D2, SuperScript II, Taq polymerase (Gibco) in a single reaction vessel with 50 [micro]L final volume.
Mud sediments beneath the structure amplified specific vibrations that matched the inherent resonant frequencies of the highway, report seismologists from the Lamont-Doherty Geological Observatory in Palisades, N.
To prevent DNA contamination and the carryover of amplified products, we used sterile tools at all times and carried out each step of the analysis (extracting DNA, preparing the reaction mixture, and amplifying and analyzing the PCR product) in separated work areas.
Mourou's group has been working with a solid-state laser--the laser signal is amplified when it passes through a piece of glass doped with neodymium.
RNA was converted to cDNA and amplified by the Titan One Tube RT-PCR System (Boehringer, Mannheim, Germany), according to the manufacturer's recommendations.

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