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Sucrose and trehalose had more considerable effects on maintaining reactivity of antigen binding sites of S3 mAbs in comparison to other additives.
Because the covalently labeled conjugate that was prepared from [F(ab').sub.2[micro]] and AP is a complex polymer molecule and has multiple antigen binding sites in one conjugate molecule, the conjugate cannot bind to the antigen at a molar ratio of 1:1.