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Immunofluorescence assay Tests in which antigen-antibody reactions are detected by fluorescence because one of the reactants is tagged with a fluorescent dye.
An antigen-antibody reaction occurs when latex particles, which bind to antigens, are mixed with a fluid containing the antibody.
The occurrence of nonspecific reactions of a magnitude similar to that of antigen-antibody reaction may be particularly misleading and requires the use of a reliable dilution protocol.
The contribution by Rodbard (5) on data handling provides a detailed treatment of an aspect of immunoassay that lives in the shadow of the huge developments in basic knowledge of the antigen-antibody reaction and reagent technology (Fig.