One case (0.86%) of 116 SCD patients in the nonalloimmunized patients developed autoantibody as revealed by positive polyclonal autocontrol and direct antiglobulin tests
Neonatal BO Incompatibility Is Associated With a Positive Cord Blood Direct Antiglobulin Test
in Infants of Black Ethnicity.
Laboratory investigations were parasitologic (thick and thin blood smears), hematologic (differential blood count), and biochemical (haptoglobin, lactate dehydrogenase [LDH], C-reactive protein, potassium, and sodium levels and renal and liver function tests) examinations; screening for glucose-6-phosphate dehydrogenase deficiency; and immunohematologic examinations (direct and indirect antiglobulin test
, including testing with enzyme-treated erythrocytes).
* Blood group incompatibility with positive direct antiglobulin test
, other known hemolytic disease (e.g.: G6PD deficiency).
Laboratory tests (increased free hemoglobin, increased lactate dehydrogenase, and decreased haptoglobin) revealed a direct antiglobulin test
(DAT)negative hemolytic anemia.
A Turkish study concluded that reticulocyte count, the presence of a sibling with neonatal jaundice and a positive direct antiglobulin test
were good predictors for the development of significant hyperbilirubinemia and a serum bilirubin levels of 4 mg/dL and 6 mg/dL at six hours of life are good predictors of severe hyperbilirubinemia.
Blood grou-ping and direct antiglobulin test
was performed on all samples.
The traditional method for the measurement of antenatal titrations is the indirect antiglobulin test
(IAT) tube method, although this method has repeatedly demonstrated imprecision in its results (1, 2).
A 10 years old child presented with progressive pallor since birth and history of jaundice and abdominal distension since 4 months.Investigations revealed a positive Direct antiglobulin test
and a diagnosis of Autoimmune hemolytic anemia was made.Steroids were given and patient responded to it as evidenced by laboratory investigations returning to normals and alleviation of jaundice,but clinically pallor persisted.
In addition, in a subset of children without hemoglobinopathies or glucose-6-phosphate dehydrogenase deficiency (n = 17), 16 were direct antiglobulin test
(DAT)-positive, and nine were positive for immunoglobulin G autoantibodies, with higher parasitemia being associated with increased DAT positivity (7).
Tu et al., "Direct antiglobulin test
of bone marrow mononuclear cells: results of 270 cases with autoimmune hemocytopenia," Chinese Journal of Practical Internal Medicine, vol.
Severe ABO hemolytic disease of the newborn with a positive direct antiglobulin test
. Indian J Pathol Microbiol.