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PCR is a technique that enables, in a test tube, the amplification of any desired specific sequence of DNA from almost any biological source to assayable quantities in a matter of hours.
The polymerase chain reaction is not a method of analysis in itself; it is a means of obtaining assayable quantities of DNA sequences from often limited source material and also speeds up the process of generating data.
Although our main study did not address the question of folate trapping, we evaluated in a pilot study whether use of an ascorbic acid diluent previously degassed with nitrogen or stirring the hemolysate for 1 h in an open container exposed to air would affect assayable folate by either LC/MS/MS or the microbiologic assay.
Absent such a belief, of course, neither the liberatory project in general, nor the university In particular are possible as cultural practices, since in that, event, institutional and political arrangements and practices are never really assayable and must be determined instead by power alone or--and this amounts to much the same thing--by groundless persuasion.
It is assumed that this procedure achieves two goals: the complete lysis of erythrocytes and the exposure of intracellular folate polyglutamates to plasma conjugase enzyme ([gamma]-glutamyl hydrolase) at a pH near 4.5, which favors their conversion to assayable folate.
Study I(a) involved an investigation of assayable RCF concentration, comparing the use of AA at pH 2.7 (the historically used diluent) with pH-adjusted AA or water, and concomitant determination of the effect of added saponin as a hemolyzing agent.
That allowing deconjugation to proceed for an excess of time, which would allow assayable folate concentration to plateau, is synonymous with ensuring complete deconjugation;