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In addition, the use of a simplified formula for calculating BT [calculated BT (cBT)] using specific association constants of testosterone for sex hormone-binding globulin (SHBG) and for albumin (3) seems to yield higher calculated than measured BT [assayed BT (aBT)] (4).
UV--Vis spectra were collected via a Hewlett-Packard 8453 UV--Vis spectrophotometer where solvents were chosen from acetonitrile, dichloromethane, or benzyl chloride, in order to determine the extinction coefficients of the ferrocene derivatives (acetonitrile) or the association constants of the ferrocene derivatives with either dichloromethane or benzyl chloride.
It is seen that the fugacity of non-associating components in the mixture depends on the association constant (K) of the associating component.
In addition, these methods assume that the association constants for [k.
Moreover, for the same samples, the CBT values did not change regardless of the optimal paired association constants [K.
In the first phase of this aim, we will synthesize neutral and cationic, mono- and bidentate candidate compounds and determine their association constants with a variety of Lewis bases.
Association constants were obtained by analyzing the individual titration experiments as described above, and the values obtained at different temperatures are given in Fig.

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