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2 0.10/38 0.26/59 -- 5.0/3 Table 2: Presence of different bacterial DNA in the samples of cosmic dust from the surface of ISS.
Real-Time Polymerase Chain Reaction for Detecting Bacterial DNA Directly from Blood of Neonates Being Evaluated for Sepsis, J Molecul Diagnost 2005; 7(5): 575-81
Bacterial DNA was extracted from the swabs by using the PowerSoil DNA Isolation kit[13].
The biotin incorporated PCR product of bacterial DNA (total 50 [micro]l) was tested in the plate according to the manufacturer instruction provided in the ELISA kit (Invitrogen-BRL-Gibco), as previously described (10).
Peter Thorne, public health professor at the UI said that their research explored a novel approach to treating mite allergy in which specially-encapsulated miniscule particles were administered with sequences of bacterial DNA that directed the immune system to suppress allergic immune responses.
To better understand the association between post-transplant microbiota and patient outcomes, the researchers collected fecal specimens from 80 patients undergoing allogeneic SCT and sequenced each sample's bacterial DNA.
(7) In clinical applications, real-time PCR for broad-range amplification of bacterial DNA offers a rapid turnaround time and a decreased risk of PCR carryover contamination, of outmost importance when using small and qualitative-poor DNA samples.
With colleagues from Baylor and Texas Children's Hospital, Aagaard analyzed 320 donated placentas, using technology that teases out bacterial DNA to evaluate the type and abundance of different microbes.
This procedure uses special probes to bind to complementary segments of bacterial DNA, which, if present, glows (fluoresces) green.
Researchers analyzed bacterial DNA from fecal samples that were collected roughly 25 years ago in a case-control study of patients with colorectal cancer.
Bacterial DNA was purified from FFPE tissue sections using a commercial kit (NucleoSpin Kit, Macherey-Nagel, Diiren, Germany) as recently described [9, 10].
These swabs were analyzed for DNA markers from more that 800 species of bacteria using bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP) and the results were compared with previously identified bacterial DNA markers.