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One possible reason for their disappointing results may have been that they measured absorbance at 490 nm (with blank correction at 630 nm) instead of at ~508 nm, the absorption maximum for the chromophores derived from cotinine and other nicotine metabolites when barbituric acid is used as the condensing reagent.
9], quantitative variants of the Konig reaction with barbituric acid as the condensing reagent performed as well among our diabetic patients as among other previously studied groups.
The reagents used in this work include N,N'-bismalei-mide-4,4'-diphenylmethane (BMI, Beil, 95%), N-phenylmaleimide (PMI, Aldrich, 97%), barbituric acid (BTA, Merk, 99%), 2,2'-azobisisobutyronitrile (AIBN, Acros, 98%), N-methyl-2-pyrrolidinone (NMP, Sigma, 99%), N,N-dimethyl formamide (DMF, TEDIA, 99.
The isothermal polymerizations of N, N' -bismaleimide-4, 4'-diphenylmethane (BMI) with barbituric acid (BTA) with BTA/BMI = 1/2 (mol [mol.