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Innovative ideas, such as improvement of bottlenecked operations, focus on value-added activities, and improvement in throughput can bring better patient care with less stress and strain on physicians and office staffs.
The levels of additive genetic variance deteriorated over the five founder-flush cycles much more rapidly for the single-pair lines than for the four- and 16-pair lines (Bryant and Meffert 1993), and thus these larger bottlenecked lines may have retained ability for continued differentiation during successive bottlenecks.
Bottlenecked lines were established from a large ancestral population by obtaining offspring from one, four, or 16 pairs of flies, respectively (with two replicate lines of each bottleneck size, designated a and b).