FA method: Using the borate buffer as the blank, and the running buffer solution
and the borate buffer with different concentrations of ODEX-BSA as the test samples, experiments were carried out using the capillary electrophoresis FA method (positive injection).
Britton--Robinson buffer solutions
(0.04 M) were prepared at pH 3.0-8.0 from 0.04 M C[H.sub.3]COOH (Merck, Germany), 0.04 M [H.sub.3]B[O.sub.3] (Aldrich, USA), and 0.04 M [H.sub.3]P[O.sub.4] (Merck, Germany).
The measurements were recorded after the addition of 100 [micro]L of lactose to the buffer solution
(pH 7.4) at 0.40 V, representing the denaturing potential for the pair of enzymes ([beta]-gal/GaOx) .
The effect of surface termination of BDD electrode on a mixture containing AML and ATOR as investigated using 30 [micro]mol [L.sup.-1] AML and 50 [micro]mol [L.sup.-1] ATOR in BR buffer solution
Tracks 1, 3, 5, 7, and 9: recTnT incubated for 3 h at 37 [degrees]C in a buffer solution
; tracks 2, 4, 6, 8, and 10: recTnT incubated for 3 h at 37 [degrees]C in a buffer solution
with thrombin (3 NIH units/mL).
The thermal stabilities of trypsin in its free and immobilized states were evaluated by measuring the residual activity at 60[degrees] C in BAEE buffer solution
(pH 8, 20 mmol/L, N[H.sub.4]HC[O.sub.3]).
After collection the rumen fluid was quickly quantified and was transferred into dark bottle containing buffer solution
at 1: 2 ratios (one part of the rumen fluid with two parts of the buffer solution
), purged with CO2 to remove oxygen and tightly closed.
A portion of fluid was used without adding buffer solution
, and another portion of fluid was buffered at a ratio of 1:2 (rumen fluid: buffer ANKON[R]).
Prior to the experiment, the sample materials were kept in buffer solution
of pH 8.0 for 24 h and subsequently vacuum dried.
Fenton R group (n = 5): 5 ml buffer solution
with lipid fraction + 1 ml FeCl2 + 1 ml 10 mM H2O2
may expire much sooner than the specified expiration dates once they are opened due to evaporation and influx of atmospheric carbon dioxide.