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These are suitable conditions for sperm cell capacitation 10 and also to evaluate their ability to undergo acrosome reaction.
Reactive oxygen species as mediators of sperm capacitation and pathological damage.
(11) Studies have shown that [O.sup.-.sub.2] production is one the first events of capacitation and the involvement of ROS generated in adenylyl cyclase and tyrosine activation is clearly demonstrated.
A shift in the balance of the oxidoreductive system is necessary to trigger the capacitation.
There are four critical steps that are involved in a successful fertilization process: sperm maturation, capacitation, hyperactivation, acrosome reaction, and sperm oocyte fusion [87, 98].
With the bulwark of their intellectual capacitation, they exemplify the hope of our nation's redemption.
Gut rhetorics, accordingly, compose bodies and microbes as ruminations, coughing up the capacitation of rhetoric, a kind of chewing, as well as chewing the cud, that is, thinking with/in the gut.
Combined Hoechst 33258/chlortetracycline fluorescence (H33258/CTC) staining is widely used method to directly measure the capacitation status of spermatozoa by monitoring calcium-regulated changes using the fluorescent antibiotic chlortetracycline [12,13].
Sperm Capacitation Is, after All, a Prerequisite for Both Partial and Complete Acrosome Reaction.
replaced with a new endocentric economic system that is based on African development capacitation, resource-based industrialization and domestic prosperity generation, Africa cannot achieve the objectives of Agenda 2063."
On the other hand, excessive ROS production lead to oxidative stress, resulting in decreased sperm motility, viability, and increased midpiece sperm defects that impair sperm capacitation and acrosome reaction .
The remaining pellet was resuspended by pipetting with 1 ml of medium and incubated at 37[degrees]C for 1 hour (capacitation).
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