Senescence is the result of cellular stresses, including DNA damage, that give rise to a
cell cycle arrest in the G1 phase of the
cell cycle; it involves the accumulation of the
cell cycle inhibitor p16INK4A.
After M phase either a cell enters in another round of
cell cycle or exits in resting phase (G0; differentiation, senescence or apoptosis) which is all well regulated.
As to the mechanisms, honokiol has antitumor activities against leukemia, breast cancer, pancreatic cancer, and oral squamous cell carcinoma cells due to induction of
cell cycle arrest and cell apoptosis (Ishikawa et al.
The cells with longer
cell cycles did in fact have shorter telomere.
Cell cycle distributions were visualized as histograms of FL2 pulse area, and the distributions of G1, S, and G2/M
cell cycle phases were modeled in Cyflogic 1.
Close analysis showed that sulforaphane induced G2/M
cell cycle arrest, higher p21 levels, cleavage of PARP and caspases (3, 8 and 9), and the inhibition of AKT/mTOR signalling in ALL cells.
Conclusion: Elevated cyclin expression during
cell cycle phases in the CML and MM patients was not associated with elevated CDKI expression.
The distribution of individual
cell cycle phases was analyzed by flow cytometry using FACSCalibur flow cytometer (BD Biosciences, San Jose, CA) for analysis of the DNA contents.
In the present study, sequences of selected genes involved in the
cell cycle pathway were selected to infer phylogeny as well as to determine their homology across various species in the animal kingdom to better understand how these genes contribute to leukemogenesis.
Sapacitabine, a
cell cycle modulating nucleoside analogue, is in phase 2 studies for the treatment of AML in the elderly, MDS and lung cancer.
CDT activity on HeLa cells was confirmed by cell distension,
cell cycle arrest by flowcytometry, and apoptosis by DNA fragmentation and flowcytometry.
The
cell cycle is an ordered series of events culminating in cell growth and division into two daughter cells.
