Caption: Figure 2: SDS-PAGE analysis of the cell-free extracts
prepared from the pcDNA4/TO, pcDNA4/TO-WT FTO and pcDNA4/TO-R316Q FTO transfected cells to demonstrate the moderate expression level of the FTO (a).
seeds was observed after exposure to low concentrations of cell-free extract
from blooms containing MC-RR (1 and 10 mg [L.sup.-1]).
Measurement of Enzymatic Activities in Cell-Free Extracts
The Program is also devoted to the development and application of powerful new imaging tools that permit such studies directly in living cells and functional cell-free extracts
. Program members have special expertise in the use of polarized light for analyzing the local arrangement of molecular bonds and fine structure in biological specimens.
In crude extracts of yeast, the sterol 14[alpha]-demethylase is inhibited by flusilazole approximately 100 times more efficiently than in crude cell-free extracts
from rat liver (Trzaskos and Henry 1989).
However, whole cells and cell-free extracts
of lactococci do not produce the compound under simulated cheese conditions in the laboratory.