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Circulating cell-surface markers that mediate innate immune and inflammatory responses were assessed by flow cytometry on each day.
However, the authors noted that labeling with CD3 was variable among the neoplastic cell populations throughout the body: not all of the neoplastic cells were positive for CD3, suggesting that some neoplastic cells had lost their characteristic cell-surface markers.
To confirm monocyte/macrophage differentiation of the treated cells under the influence of 3-HK and/or the new compound, the expression of cell-surface markers, such as CD11b and CD14, was also studied.