21] The muscularis mucosa is still visible and quite intact, but its surface is starting to erode, the adipose tissues are starting to invade, the chief cells
are degrading, and the parietal cells are redilating due to necrosis.
Photomicrograph showing chief cells
, which are focally immunoreactive for tyrosine hydroxylase (original magnification X20).
C(1984) (26) that oxyphil cells may represent a non-secretary stage in life cycle of chief cells
Based on effective TPEF of cellular nicotinamide adenine dinucleotide phosphate and flavin adenine dinucleotide and the lack of TPEF of mucin and cellular nuclei, MPM can easily identify different types of cells, such as squamous epithelial cells, goblet cells, foveolar gastric mucous neck cells, parietal cells, and chief cells
Mills, graduate student Greg Sibbel and Hans Clevers, a geneticist at Utrecht Medical Center, identified markers that show a small number of chief cells
become stem cells even in the absence of serious injury.
Paragangliomas are composed of two cell types: chief cells
and sustentacular cells.
26,28) These are composed of chief cells
and sustentacular cells in a nesting or zellballen pattern.
Immunohistochemistry confirmed the presence of 2 cell populations: the chief cells
stained with neuron-specific enolase and chromogranin, whereas the sustentacular cells stained with S100 protein.
Histologically, these tumors appear highly vascular and are composed of clusters (Zellballen, which is German for "cell balls") of chief cells
supported by sustentacular cells and small blood vessels.
Histopathologic examination revealed that parts of the tumor contained a lobulated architecture composed of sheets, cords, and follicles of chief cells
, oxyphil cells, and clear cells; these findings were consistent with an adenoma.