21] The muscularis mucosa is still visible and quite intact, but its surface is starting to erode, the adipose tissues are starting to invade, the
chief cells are degrading, and the parietal cells are redilating due to necrosis.
Photomicrograph showing
chief cells, which are focally immunoreactive for tyrosine hydroxylase (original magnification X20).
C(1984) (26) that oxyphil cells may represent a non-secretary stage in life cycle of
chief cells.
Based on effective TPEF of cellular nicotinamide adenine dinucleotide phosphate and flavin adenine dinucleotide and the lack of TPEF of mucin and cellular nuclei, MPM can easily identify different types of cells, such as squamous epithelial cells, goblet cells, foveolar gastric mucous neck cells, parietal cells, and
chief cells.
Mills, graduate student Greg Sibbel and Hans Clevers, a geneticist at Utrecht Medical Center, identified markers that show a small number of
chief cells become stem cells even in the absence of serious injury.
Paragangliomas are composed of two cell types:
chief cells and sustentacular cells.
26,28) These are composed of
chief cells and sustentacular cells in a nesting or zellballen pattern.
Immunohistochemistry confirmed the presence of 2 cell populations: the
chief cells stained with neuron-specific enolase and chromogranin, whereas the sustentacular cells stained with S100 protein.
Histologically, these tumors appear highly vascular and are composed of clusters (Zellballen, which is German for "cell balls") of
chief cells supported by sustentacular cells and small blood vessels.
Histopathologic examination revealed that parts of the tumor contained a lobulated architecture composed of sheets, cords, and follicles of
chief cells, oxyphil cells, and clear cells; these findings were consistent with an adenoma.
