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aeruginosa using ammonium sulphate precipitation and DEAE cellulose column chromatography.
reflexa which was present in sufficient amount was used for column chromatography to obtain the fractions.
Removal of methanol by distillation and addition of water followed by extraction with ethyl acetate gave crude alkylation product 1, which was purified by rapid column chromatography followed by crystallization from CH[Cl.
The fungal mycelia were then separated from the broth by filtration, and the culture broth was then subjected to aromatic absorbent resin column chromatography using Dianion HP-20 and elutecl with a step gradient of [H.
The solvent was removed under reduced pressure and the residue was purified by column chromatography through silica gel using as an eluent a mixture of n-hexane and ethyl acetate (9:1) to give 7 (1.
All column chromatography was carried out using ACROS silica gel 0.
Readers are assumed to have knowledge of synthetic reactions and techniques; analytical methods such as TLC, NMR, UV spectroscopy, and mass spectrometry; and standard laboratory techniques such as solvent extraction and column chromatography.
High-performance liquid chromatography (or high pressure liquid chromatography, HPLC) is a form of column chromatography used to separate, identify, and quantify ionic and organic compounds.
Methods: The FPLC method, using a DEAE Sepharose, Hi Trap[TM] anion-exchange column chromatography technique was set up for [HbA.
Column chromatography was used to purify and isolate the product, which was characterized by 1H NMR and Electrospray Ionization Mass Spectrometry.
The OchraTest WB, as it is called, works by using immunoaffinity column chromatography to isolate and detect ochratoxin.