These findings confirmed that RT-PCR is more reliable in detecting BEFV than complement fixation test
 for slaughterhouse workers in Sao Luis, Maranhao, where a prevalence of 2.2% was reported by the Complement fixation test
. Spinola & Costa  in Salvador, Bahia, have studied human brucellosis under the serological, occupational and clinic perspective in 128 cold storage plant workers and reported a prevalence of 10.6% in the test of fast and slow seroagglutination.
Serum samples from this dromedary tested positive for glanders with both the OIE-acknowledged complement fixation test
(titer 10+++) and with the Central Veterinary Research Laboratory-developed in-house competitive ELISA (7) with an inhibition of 57%.
The complement fixation test
(CFT) and the highly specific and newly validated immunoblot (IB) technique were used for serodiagnosis.
The organism is bigger than a rickettsia, however, and the convalescent guinea pig sera failed to react in the complement fixation test
with standard rickettsial antigens prepared from Coxiella burnetii, Rickettsia rickettsii, R.
The virus was identified by complement fixation test
and confirmed by reverse transcription--polymerase chain reaction.
Results of several scroprevalence studies, using the complement fixation test
, conducted in both humans and animals suggest that C.