Comparison between estimated sperm count and Neubauer counting chamber
recordings Group 1, estimated count Group 2, estimated count < 40 sperm (N=14) 40-100 sperm (N=14) Estimated Neubauer count, Estimated Neubauer count, count x [10.sup.6]/mL count x [10.sup.6]/mL Mean 20.0 2.5 71 16.0 SD 8.0 1.2 15.3 10.4 Range 8.0-34 4-Jan 15-90 6-34 Group 3, estimated count > 101 sperm (N=23) Estimated Neubauer count, count x [10.sup.6]/mL Mean 100.0 48.3 SD 0.0 21.7 Range 100 21-98 Table 2.
This retrieval seems unlikely in other methods including Coulter counter image based counters flow cytometry and counting chambers
Manual microscopic examination was performed in Fuchs-Rosenthal counting chambers
. For each sample, at least 20 random microscopic fields were examined at x400, and the mean number of cells/[mm.sup.3] was calculated.
Pyuria: deposit in high-power microscopic field WBC/HPF versus WBC/mm in counting chamber
. Acta Med Scand 1973; 194:537-40.
Better correlations between CD and the counting chamber
for CSF samples high in lymphocytes were achieved when raw uncorrected values measured on the CD were used.
Total Leukocyte count (TLC) was done using Improved Neubauer's Counting chamber
. Absolute counts were obtained by doing Differential Leukocyte Count (DLC) and the value obtained from TLC.
The leucocytes were counted in squares present at the four corners of the counting chamber
The total leucocyte count was done using 0.5 part of blood mixed with 10 part of Turk's fluid followed by counting of leucocyte in the counting chamber
(improved Neubauer's chamber) under light microscope.
(1) Cell counts should be performed in a hemocytometer counting chamber
or in an automated cell-counting instrument if the volume and cellularity is great enough.
The frozen aliquots allow the controls to be analyzed in a counting chamber
, but due to the variation in sperm-cell death during thawing, the motility of frozen-thawed sperm is highly variable.
"In order to fully appreciate the Coulter Counter, one needs to view its significance in relation to the medical technology of its day--in this case, the hundred-year-old method of visually counting cells with a counting chamber
and microscope," says Alan Jones, MD, who became friends with Wallace Coulter when Wallace personally installed an early Coulter Counter model in Jones' laboratory at Children's Medical Center in Boston in 1958.