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Detection of MAC through urine culture and/or polymerase chain reaction (PCR) is most commonly used to establish the diagnosis, though direct staining for acid-fast bacilli has also been used, but is thought to be unreliable.
Similarly, some authors have reported diagnosis of severe DH by using direct staining of skin samples (4); in low-income countries, skin involvement is the main presentation of DH because of limited laboratory facilities and/or late diagnosis.
35) described a method for rapidly detecting biofilm cells on catheters by direct staining of the catheter with acridine orange.