In vivo embryoids
from anthers of Narcissus biflorus Curt.
6] individual embryonic stem cells were plated at low density in bacteriological petri dishes for embryoid
10) This was done by initiating the differentiation of embryonic stem cells into aggregates called embryoid
Effects of media on embryoid
induction and plant regeneration from cultured anthers of soft white spring wheats (Triticum aestivum L.
They were used to create multicellular structures called embryoid
bodies, which have some of the characteristics of early embryos and the beginnings of differentiated tissue.
The researchers started by coaxing embryonic stem cells to form clusters known as embryoid
They can spontaneously form embryoid
bodies, clusters of differentiated cells also formed by embryonic stem cells.
16 Determining Pluripotency of Human Embryonic Stem Cells: Embryoid
body formation and in vitro differentiation
For instance, they can differentiate in dishes as three dimensional aggregates called embryoid
bodies resembling the early stages of embryonic development.
One possible method is spontaneous differentiation in adherent culture after removing factors that promote pluripotency as feeders and Leukemia Inhibitory Factor (LIF) or basic Fibroblast Growth Factor (bFGF) (16), (18) whereas the second one implicates the formation of three-dimensional structures known as Embryoid
Bodies (EBs) (13), (19), (20).
Researchers are now reporting advances in these areas by using gelatin-based microparticles to deliver growth factors to specific areas of embryoid
bodies, aggregates of differentiating stem cells.