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In the spontaneous differentiation study, embryoid bodies lost their normal morphology at day 8 of suspension culture and after attachment they differentiated into various types of cells.
They were allowed to mature for two or three weeks inside the embryoid bodies before being located and removed.
They can spontaneously form embryoid bodies, clusters of differentiated cells also formed by embryonic stem cells.
They were used to create multicellular structures called embryoid bodies, which have some of the characteristics of early embryos and the beginnings of differentiated tissue.
The researchers started by coaxing embryonic stem cells to form clusters known as embryoid bodies.