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41, 42) However, experimental studies on fluorosis have focused specifically on the stage of enamel maturation (which includes the orderly sequence of crystal growth, proteolytic digestion by different enzymes, and absorption of protein residues), as it has proven to be the most sensitive to the negative effects of F-.
Suckling [1980] suggested that opacities are a qualitative defect of the matrix formed by disorders involving ameloblasts during the stage of enamel maturation.
During normal enamel maturation, there is an increase in mineralization and a loss of matrix proteins.