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Use of frozen sera for FT4 standardization: investigation by equilibrium dialysis combined with isotope dilution mass spectrometry and immunoassay.
Based on these clinical and laboratory findings, the free thyroxine level was measured by equilibrium dialysis and found to be low.
The patent issued to Harvard Bioscience is for an equilibrium dialysis system that enables samples to be prepared simultaneously in multiples.
Free thyroid hormones in serum by direct equilibrium dialysis and online solid-phase extraction-liquid chromatography/ tandem mass spectrometry.
1] Nonstandard abbreviations: UF, ultrafiltration; EDIA, equilibrium dialysis immunoassay; UFMSMS, UF tandem mass spectrometry; FT4, free thyroxine; [FT.
4] measured by equilibrium dialysis (EDFT4) was within the reference interval (Table 1).
In the field of free thyroid hormone testing, methods based on equilibrium dialysis (ED) or ultrafiltration (UF) of serum, before direct measurement of the hormone concentration in the dialysate or ultrafiltrate, are accepted to be gold standards (1).
Theoretically, both equilibrium dialysis (ED) and ultrafiltration (UF) can be valid methodologies.
It is of note that the reference interval for free T by analog-based assay is much lower (concentrations about one-fifth as high) than that for the equilibrium dialysis assay (2).

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