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Feeder layer cells were essential for cells fusion and increased C4H5 hybridoma growth because they provided numerous growth factors and cleared dead myeloma cells, splenocytes, and cell debris by phagocytosis; they also conveyed antimicrobial activity (Smith, 2001; Li et al.
Based on previous studies,11 we decided to use Sertoli cells as a feeder layer in addition to growth factors in vitro to create a testis-like microenvironment to better support the Sertoli cells and SSCs.
Choosing a suitable endodermal differentiation medium and feeder layer is an important step for obtaining safe and efficient endoderm cells, especially for their use in the differentiation into the cells used for cell replacement therapy.
Culturing of keratinocytes is not easy as traditionally there was use of feeder layer to grow keratinocytes and there was rapid apoptosis in cells without feeder layer (Rheinwald and Green, 1975; Tenchini et al.
Several lines of evidence suggest that the ex vivo expanded limbal epithelial cells on the intact HAM with the feeder layer are able to preserve the characteristics of limbal epithelial cells (7-9).
Currently, irradiated mouse embryonic fibroblast feeder layers are utilized, but it is possible that other tissues would be used in the future.
The differentiation protocol does not require serum or feeder layer cells and therefore allows scalable production of hESC-derived islet-like cells, an important condition for therapeutic development.
Royan B1, mESCs derived from the C57BL/6 strain of mouse (26) were grown on a feeder layer of the primary Mouse Embryonic Fibroblasts (MEF) in tissue culture flasks.
connexin 43 and keratin 3 / keratin 12) on the limbal epithelial cells cultured over the denuded,AM with and without the 3T3 murine fibroblast cells as feeder layer.
In this study, scientists generated human iPS cell lines by using a method in which the feeder layer secretes a protein called LIF.