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To demonstrate the specific IgY immunoglobulin activity, an Ouchterlony double gel diffusion test was used [19].
Single radial gel diffusion by using the [beta]-D-glucosyl-Yariv (Me gazyme, Sydney, Australia) antigen was performed according to the procedure of Holst and Clarke (1985).
The double gel diffusion precipitin (Ouchterlony) test has been used for quite some time.