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Assessment of HER-2/neu gene amplification by FISH technique and HER-2/neu oncoprotein overexpression by immunohistochemistry should be done in all patients with gastric and gastro-oesophageal carcinoma so that these patients can be benefitted from molecularly targeted therapy i.
R175H mutation and 10% for MYC and ERBB2 gene amplification, respectively (see online Supplemental Table 6).
All primary breast cancer cases accrued to our University of Southern California (Los Angeles, California) Breast Cancer Analysis Laboratory consultation practice from April 1999 until September 2015 that had both HER2 gene amplification status determined by FISH and HER2 protein level determined by IHC were eligible for inclusion in this study.
This PCR method was also used for detection of gene amplification of other Erb oncogenes such as ErbB1 in cancer tissues (16).
Dual-color silver-enhanced in situ hybridization for assessing HER2 gene amplification in breast cancer.
Detection of gene amplification in MYCN, C-MYC, MYCL1, ERBB2, EGFR, AKT2, and human papilloma virus in samples from cervical smear normal cytology, intraepithelial cervical neoplasia (CIN I, II, III), and cervical cancer
8) In about 3% of the cases, over expression of HER2/neu can occur in the absence of gene amplification giving rise to false positive results on IHC and negative by
The discovery of targeted therapy against the HER2 gene in the form of the humanized anti-HER2 monoclonal antibody trastuzumab (Herceptin, Genentech, South San Francisco, CA) and HER1/HER2 dual receptor inhibitor, lapatinib, has brought forward an effective treatment modality for patients having the gene amplification (4-6).
HER2/neu gene amplification and protein overexpression occurs in approximately 15-20% of invasive breast cancers and is associated with poor disease-free survival and resistance to certain chemotherapeutic agents.
The two primary gene cloning techniques are then described: polymerase chain reaction (PCR) for gene amplification and an RT-PCR cloning strategy for lipase cloning from a microbial source.